Linkage Mapping and Comparative Genomics of Red Drum (Sciaenops ocellatus) Using Next-Generation Sequencing

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Linkage Mapping and Comparative Genomics of Red Drum (Sciaenops ocellatus) Using Next-Generation Sequencing

Developments in next-generation sequencing allow genotyping of thousands of genetic markers across hundreds of individuals in a cost-effective manner. Because of this, it is now possible to rapidly produce dense genetic linkage maps for nonmodel species. Here, we report a dense genetic linkage map for red drum, a marine fish species of considerable economic importance in the southeastern United...

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A genetic linkage map of red drum, Sciaenops ocellatus.

Second-generation, sex-specific genetic linkage maps were generated for the economically important estuarine-dependent marine fish Sciaenops ocellatus (red drum). The maps were based on F(1) progeny from each of two single-pair mating families. A total of 237 nuclear-encoded microsatellite markers were mapped to 25 linkage groups. The female map contained 226 markers, with a total length of 127...

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Linkage Mapping and Comparative Genomics Using Next-Generation RAD Sequencing of a Non-Model Organism

Restriction-site associated DNA (RAD) sequencing is a powerful new method for targeted sequencing across the genomes of many individuals. This approach has broad potential for genetic analysis of non-model organisms including genotype-phenotype association mapping, phylogeography, population genetics and scaffolding genome assemblies through linkage mapping. We constructed a RAD library using g...

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Ontogeny of critical swimming speed of wild-caught and laboratory-reared red drum Sciaenops ocellatus larvae

Critical swimming speed (Ucrit) provides a useful estimate of maximum swimming performance for fish larvae that can be used to assess transport and migratory potential. We measured Ucrit of red drum Sciaenops ocellatus larvae through its ontogeny and compared the swimming performance of laboratory-reared larvae to that of wild-caught individuals. Ucrit increased with ontogeny (size), even thoug...

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ژورنال

عنوان ژورنال: G3 Genes|Genomes|Genetics

سال: 2017

ISSN: 2160-1836

DOI: 10.1534/g3.116.036350